Isolation and characterization of microsatellite loci for mountain mullet (Agonostomus monticola)

Patrick J. Sanchez, University of Southern Mississippi
Wilfredo A. Matamoros, University of Southern Mississippi
Jacob F. Schaefer, University of Southern Mississippi
Brian R. Kreiser, University of Southern Mississippi

Abstract

We report on the isolation of 15 polymorphic microsatellite loci from mountain mullet (Agonostomus monticola). In the two populations sampled, loci exhibited two to 21 alleles and observed heterozygosity values ranged from 0.222 to 1.000. All loci conformed to Hardy-Weinberg equilibrium expectations, and none exhibited linkage disequilibrium. Although A. monticola is an important subsistence fishery in parts of its range, little is known about its ecology and many populations appear to be experiencing declines. These microsatellite loci should prove useful in the study of population structure of A. monticola and aid in other potential conservation efforts such as the management of hatchery broodstock.