A filter-based propidium monoazide technique to distinguish live from membrane-compromised microorganisms using quantitative PCR

Shiao Y. Wang, University of Southern Mississippi

Abstract

Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings. (C) 2012 Elsevier B.V. All rights reserved.