Evaluation of an Alkaline Phosphatase-Labeled Oligonucleotide Probe for the Detection and Enumeration of the Thermostable-Related Hemolysin (trh) Gene of Vibrio parahaemolyticus
Coastal Sciences, Gulf Coast Research Laboratory
Reliable methods are needed to detect total and pathogenic Vibrio parahaemolyticus. One marker of V. parahaemolyticus virulence is the thermostable-related hemolysin. We developed an alkaline phosphatase-labeled DNA probe method for the specific detection and enumeration of trh-positive V. parahaemolyticus by colony hybridization. The probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive V. parahaemolyticus. Additionally, the trh alkaline phosphatase probe colony hybridization was successfully used to detect and enumerate trh-positive V. parahaemolyticus in seafood and water samples collected from the United States and the United Kingdom.
Journal of Food Protection
Nordstrom, J. L.,
Vickery, M. C.,
Phillips, A. M.,
Murray, S. L.,
(2006). Evaluation of an Alkaline Phosphatase-Labeled Oligonucleotide Probe for the Detection and Enumeration of the Thermostable-Related Hemolysin (trh) Gene of Vibrio parahaemolyticus. Journal of Food Protection, 69(11), 2770-2772.
Available at: http://aquila.usm.edu/fac_pubs/2185