Synthesis, Characterization, and Antibacterial Activities of Novel Methacrylate Polymers Containing Norfloxacin
Document Type
Article
Publication Date
1-1-2005
Department
Polymers and High Performance Materials
Abstract
A novel methacrylate monomer containing a quinolone moiety was synthesized and homopolymerized in N,N-dimethylformamide (DMF) by using azobisisobutyronitrile (AIBN) as an initiator. The new monomer was copolymerized with poly(ethylene glycol) methyl ether methacrylate (MPEGMA) in DMF using the same initiator. The monomer, homopolymer, and copolymer were characterized by elemental analysis, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), size exclusion chromatography (SEC), FTIR, 13C NMR, and 1H NMR. The antibacterial activities of the monomer as well as polymers were investigated against Staphylococcus aureus and Escherichia coli, which are representative of Gram-positive and Gram-negative bacteria, respectively. All compounds showed excellent antibacterial activities against these two types of bacteria. The antibacterial activities were determined using the shaking flask method, where 25 mg/mL concentrations of each compound were tested against 105 CFU/mL bacteria solutions. The number of viable bacteria was calculated by using the spread plate method, where 100 μL of the incubated antibacterial agent in bacteria solutions were spread on agar plates and the number of viable bacteria was counted after 24 h of incubation period at 37 °C.
Publication Title
Biomacromolecules
Volume
6
Issue
1
First Page
514
Last Page
520
Recommended Citation
Dizman, B.,
Elasri, M. O.,
Mathias, L. J.
(2005). Synthesis, Characterization, and Antibacterial Activities of Novel Methacrylate Polymers Containing Norfloxacin. Biomacromolecules, 6(1), 514-520.
Available at: https://aquila.usm.edu/fac_pubs/2896