Procedure for Selecting Monoclonal Antibodies for Use in a Ligand Displacement Assay of Serum Antibody Levels

Document Type

Article

Publication Date

1991

Department

Chemistry and Biochemistry

School

Mathematics and Natural Sciences

Abstract

Monoclonal antibodies for use in a ligand displacement assay were selected for specificity and affinity/avidity properties that result in their release and displacement in the presence of specific sample antibody but not in the presence of antibodies against other antigens. A screening process is described which involves measurement of displacement of antibody by an ELISA procedure using an enzyme labeled anti-immunoglobulin, providing a means of demonstrating usefulness of a candidate antibody in a ligand displacement format without necessitating the production of enzyme conjugates of each candidate antibody to be screened. The procedure was used to screen a set of eleven monoclonal antibodies (initially selected for anti-Trichinella spiralis specificity by conventional screening methods), and successfully discriminated between antibodies which were useful in the ligand displacement format and those which were not.

Publication Title

Journal of Immunoassay

Volume

12

Issue

1

First Page

125

Last Page

144

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