SCREENING A YEAST PROMOTER LIBRARY LEADS TO THE ISOLATION OF THE RP29/L32 AND SNR17B/RPL37A DIVERGENT PROMOTERS AND THE DISCOVERY OF A GENE ENCODING RIBOSOMAL PROTEIN-L37

GM SANTANGELO
J TORNOW
CS MCLAUGHLIN
K MOLDAVE

Abstract

Two promoters (A7 and A23), isolated at random from the Saccharomyces cerevisiae genome by virtue of their capacity to activate transcription, are identical to known intergenic bidirectional promoters. Sequence analysis of the genomic DNA adjacent to the A7 promoter identified a split gene encoding ribosomal (r) protein L37, which is homologous to the tRNA-binding r-proteins, L35a (from human and rat) and L32 (from frogs).