PHOTOSYSTEM-2 REMAINS CAPABLE OF OXYGEN EVOLUTION AFTER LOSING 1/4 OF ITS INITIAL MANGANESE CONTENT
Chemistry and Biochemistry
Photosystem 2 (PS 2) membranes were treated with CaCl2 to remove extrinsic polypeptides. After resuspension in a chloride-free buffer, manganese was extracted by leaching or with EDTA. Measurements made using three different methods (electron paramagnetic resonance, kinetics, and direct Mn analysis) agreed that one-fourth of the initial Mn content was lost and that the membrane preparation remained homogenous. Upon readdition of extrinsic polypeptides, the Mn-depleted membranes were capable of oxygen evolution. Thus not all the Mn in PS 2 is necessary for the oxygen evolution function.
(1991). PHOTOSYSTEM-2 REMAINS CAPABLE OF OXYGEN EVOLUTION AFTER LOSING 1/4 OF ITS INITIAL MANGANESE CONTENT. PHOTOSYNTHETICA, 25(4), 631-638.
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