Title

PHOTOSYSTEM-2 REMAINS CAPABLE OF OXYGEN EVOLUTION AFTER LOSING 1/4 OF ITS INITIAL MANGANESE CONTENT

Document Type

Article

Publication Date

1991

Department

Chemistry and Biochemistry

Abstract

Photosystem 2 (PS 2) membranes were treated with CaCl2 to remove extrinsic polypeptides. After resuspension in a chloride-free buffer, manganese was extracted by leaching or with EDTA. Measurements made using three different methods (electron paramagnetic resonance, kinetics, and direct Mn analysis) agreed that one-fourth of the initial Mn content was lost and that the membrane preparation remained homogenous. Upon readdition of extrinsic polypeptides, the Mn-depleted membranes were capable of oxygen evolution. Thus not all the Mn in PS 2 is necessary for the oxygen evolution function.

Publication Title

PHOTOSYNTHETICA

Volume

25

Issue

4

First Page

631

Last Page

638

This document is currently not available here.

Find in your library

Share

 
COinS