Title

Chloroplast and Mitochondrial: DNA Polymerases From Cultured Soybean Cells

Document Type

Article

Publication Date

4-1-1990

Department

Chemistry and Biochemistry

Abstract

DNA polymerases were purified from chloroplasts and mitochondria of cultured Glycine max cells. The chloroplast enzyme exists in two forms which are indistinguishable from each other biochemically. All three organellar enzymes have an estimated molecular weight of 85,000 to 90,000 and prefer poly(rA)dT12-18 over activated DNA as a template in vitro. Maximum activity of the chloroplast and mitochondrial DNA polymerases requires KCI and a reducing agent, and the enzymes are completely resistant to inhibitors of DNA polymerase a. Taken together, these properties classify the soybean organellar enzymes as DNA polymerases y. A unique feature that distinguishes the plant enzymes from their animal counterparts is their resistance to dideoxyribonucleotides.

Publication Title

Plant Physiology

Volume

92

Issue

4

First Page

939

Last Page

945