Partial Purification and Characterization of a DNA Helicase from Chloroplasts of Glycine Max
Chemistry and Biochemistry
A DNA helicase activity was detected in extracts of purified chloroplasts from the SB-1 cell line of Glycine max and partially purified by column chromatography on DEAE cellulose, phosphocellulose, and single-stranded DNA cellulose. The chloroplast helicase has a DNA-dependent ATPase activity, and its strand displacement activity is strictly dependent upon the presence of a nucleoside triphosphate and Mg2+ or Mn2+. Strand displacement activity does not require a free unannealed single-strand or replication fork-like structure.
Plant Molecular Biology
Cannon, G. C.,
(1990). Partial Purification and Characterization of a DNA Helicase from Chloroplasts of Glycine Max. Plant Molecular Biology, 15(3), 457-464.
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