Title

A Filter-Based Propidium Monoazide Technique to Distinguish Live from Membrane-Compromised Microorganisms Using Quantitative PCR

Document Type

Article

Publication Date

4-1-2012

Department

Biological Sciences

Abstract

Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings. (C) 2012 Elsevier B.V. All rights reserved.

Publication Title

Journal of Microbiological Methods

Volume

89

Issue

1

First Page

76

Last Page

78