Title

Linking Probe Thermodynamics to Microarray Quantification

Document Type

Article

Publication Date

12-1-2010

Department

Coastal Sciences, Gulf Coast Research Laboratory

Abstract

Understanding the difference in probe properties holds the key to absolute quantification of DNA microarrays. So far, Langmuir-like models have failed to link sequence-specific properties to hybridization signals in the presence of a complex hybridization background. Data from washing experiments indicate that the post-hybridization washing has no major effect on the specifically bound targets, which give the final signals. Thus, the amount of specific targets bound to probes is likely determined before washing, by the competition against nonspecific binding. Our competitive hybridization model is a viable alternative to Langmuir-like models.

Publication Title

Physical Biology

Volume

7

Issue

4