Reproductive Function for a C-terminus Extended, Male-Transmitted Cytochrome c Oxidase Subunit II Protein Expressed In Both Spermatozoa and Eggs

Authors

Rumela Chakrabarti, State University of New YorkFollow
Jennifer M. Walker, University of Southern MississippiFollow
E.G. Chapman, Kent State UniversityFollow
S.P. Shepardson, Saginaw Valley State University
R.J. Trdan, Saginaw Valley State University
J.P. Curole, University of Southern California
G.T. Watters, Ohio State University
D.T. Stewart, Acadia UniversityFollow
S. Vijayaraghavan, Kent State UniversityFollow
W.R. Hoeh, Kent State UniversityFollow

Document Type

Article

Publication Date

11-13-2007

Department

Biological Sciences

School

Biological, Environmental, and Earth Sciences

Abstract

Our previous study documented expression of a male-transmitted cytochrome c oxidase subunit II protein (MCOX2), with a C-terminus extension (MCOX2e), in unionoidean bivalve testes and sperm mitochondria. Here, we present evidence demonstrating that MCOX2 is seasonally expressed in testis, with a peak shortly before fertilization that is independent of sperm density. MCOX2 is localized to the inner and outer sperm mitochondrial membranes and the MCOX2 antibody’s epitope is conserved across >65 million years of evolution. We also demonstrate the presence of male-transmitted mtDNA and season-specific MCOX2 spatial variation in ovaries. We hypothesize that MCOX2 plays a role in reproduction through gamete maturation, fertilization and/or embryogenesis.

Comments

© 2007. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Publication Title

FEBS Letters

Volume

581

Issue

27

First Page

5213

Last Page

5219

Recommended Citation

Chakrabarti, R., Walker, J. M., Chapman, E., Shepardson, S., Trdan, R., Curole, J., Watters, G., Stewart, D., Vijayaraghavan, S., Hoeh, W. (2007). Reproductive Function for a C-terminus Extended, Male-Transmitted Cytochrome c Oxidase Subunit II Protein Expressed In Both Spermatozoa and Eggs. FEBS Letters, 581(27), 5213-5219.
Available at: https://aquila.usm.edu/fac_pubs/8487