Title

Isolation and Characterization of Microsatellite Markers in Fraser fir (Abies fraseri)

Document Type

Article

Publication Date

3-1-2006

Department

Biological Sciences

Abstract

We describe the isolation and characterization of 14 microsatellite loci from Fraser fir (Abies fraseri). These markers originated from cloned inserts enriched for DNA sequences containing tandem di- and tri-nucleotide repeats. In total, 36 clones were selected, sequenced and evaluated. Polymerase chain reaction (PCR) primers for 14 of these sequences consistently produced simple PCR profiles and were found to be polymorphic among 13 Fraser fir samples. In addition, more than half of these loci were found to amplify a wide range of samples from several Abies taxa.

Publication Title

Molecular Ecology Notes

Volume

6

Issue

1

First Page

65

Last Page

68