Title

Synthesis of Symmetrical Thiol-Adenosine conjugate and 5 ' thiol-RNA Preparation by Efficient One-Step Transcription

Document Type

Article

Publication Date

11-1-2010

Department

Chemistry and Biochemistry

Abstract

A symmetrical transcription initiator containing two adenosines and conjugated thiol functionality (ThioAMP dimer) is chemically synthesized. Transcription in the presence of ThioAMP dimer under the T7 Phi 2.5 promoter yields 5' thiol-labeled RNA (5' HS-RNA) with up to 90% labeling efficiency, depending on the concentration ratio of ThioAMP dimer to ATP. The resulting 5' HS-RNA may be used directly or after thiopropyl Separose 6B affinity column purification. Biotinylation of 5' HS-RNA and formation of gold nanoparticle-RNA nanoplexes are demonstrated. (C) 2010 Elsevier Ltd. All rights reserved.

Publication Title

Bioorganic and Medicinal Chemistry Letters

Volume

20

Issue

21

First Page

6254

Last Page

6257