A Simple and Efficient Method to Prepare Thioesters in Aqueous Solutions
Chemistry and Biochemistry
A simple method is described for the efficient synthesis of biologic,ally-active thioesters in aqueous solutions. The method utilizes imidazole as a catalyst and easily synthesized acyl or aminoacyl adenylates to synthesize a variety of thioesters, from small molecules to macromolecules. Yields in excess of 90% can be achieved in less than 10 min at room temperature. Specifically, functional derivatization of RNA with biotin via thioester linkage is demonstrated. (c) 2005 Elsevier Ltd. All rights reserved.
(2005). A Simple and Efficient Method to Prepare Thioesters in Aqueous Solutions. Tetrahedron Letters, 46(25), 4307-4310.
Available at: http://aquila.usm.edu/fac_pubs/9074