Novel Cyanine-AMP Conjugates for Efficient 5' RNA Fluorescent Labeling by One-Step Transcription and Replacement of [γ-³²P] ATP in RNA Structural Investigation

Authors

Na Li, University of Southern MississippiFollow
Changjun Yu, AdeGenix, Inc.
Faqing Huang, University of Southern MississippiFollow

Document Type

Article

Publication Date

1-1-2005

Department

Chemistry and Biochemistry

School

Mathematics and Natural Sciences

Abstract

Two novel fluorescent cyanine-AMP conjugates, F550/570 and F650/670, have been synthesized to serve as transcription initiators under the T7 φ2.5 promoter. Efficient fluorophore labeling of 5′ RNA is achieved in a single transcription step by including F550/570 and F650/670 in the transcription solution. The current work makes fluorescently labeled RNA readily available for broad applications in biochemistry, molecular biology, structural biology and biomedicine. In particular, site-specifically fluorophore-labeled large RNAs prepared by the current method may be used to investigate RNA structure, folding and mechanism by various fluorescence techniques. In addition, F550/570 and F650/670 may replace [γ-³²P]ATP to prepare 5′ labeled RNA for RNA structural and functional investigation, thereby eliminating the need for the unstable and radio-hazardous [γ-³²P]ATP.

Comments

Originally published in Nucleic Acids Research, 2005, Vol. 33, No. 4

Publication Title

Nucleic Acids Research

Volume

33

Issue

4

First Page

1

Last Page

8

Recommended Citation

Li, N., Yu, C., Huang, F. (2005). Novel Cyanine-AMP Conjugates for Efficient 5' RNA Fluorescent Labeling by One-Step Transcription and Replacement of [γ-³²P] ATP in RNA Structural Investigation. Nucleic Acids Research, 33(4), 1-8.
Available at: https://aquila.usm.edu/fac_pubs/9087