EPA Gulf of Mexico Alliance MST Study
This protocol outlines the procedure developed for the EPA Gulf of Mexico grant to Harwood, Lepo and Wang to process environmental water samples via DNA extraction from membrane filters. DNA is then used in PCR-based microbial source tracking assays for human-associated Bacteroides (HBac), M. smithii (Msmithii) and human polyomavirus BK and JC (HPyVs) targets.
Microbial Source Tracking, "Standard Protocols for the 3 MST Markers Evaluated" (2010). EPA Study. Paper 2.