Supporting Data for the Characterization of PNA-DNA Four-Way Junctions

Authors

Douglas Van Iverson II, University of Southern MississippiFollow
Crystal C. Serrano, University of Southern MississippiFollow
Ann Marie Brahan, University of Southern MississippiFollow
Arik Shams, University of Southern Mississippi
Filbert Totsingan, New York UniversityFollow
Anthony J. Bell Jr., University of Southern MississippiFollow

Document Type

Article

Publication Date

12-2015

Department

Chemistry and Biochemistry

School

Mathematics and Natural Sciences

Abstract

Holliday or DNA four-way junctions (4WJs) are cruciform/bent structures composed of four DNA duplexes. 4WJs are key intermediates in homologous genetic recombination and double-strand break repair. To investigate 4WJs in vitro, junctions are assembled using four asymmetric DNA strands. The presence of four asymmetric strands about the junction branch point eliminates branch migration, and effectively immobilizes the resulting 4WJ. The purpose of these experiments is to show that immobile 4WJs composed of DNA and peptide nucleic acids (PNAs) can be distinguished from contaminating labile nucleic acid structures. These data compare the electrophoretic mobility of hybrid PNA–DNA junctions vs. i) a classic immobile DNA 4WJ, J1 and ii) contaminating nucleic acid structures.

Comments

Published by Data in Brief at 10.1016/j.dib.2015.10.013.

Publication Title

Data in Brief

Volume

5

First Page

756

Last Page

760

Recommended Citation

Iverson, D. V., Serrano, C. C., Brahan, A. M., Shams, A., Totsingan, F., Bell, A. J. (2015). Supporting Data for the Characterization of PNA-DNA Four-Way Junctions. Data in Brief, 5, 756-760.
Available at: https://aquila.usm.edu/fac_pubs/15412