Document Type

Article

Publication Date

10-21-2016

Department

Biological Sciences

Abstract

Block ionomer complex (BIC)–siRNA interactions and effectiveness in cell transfection are reported. Aqueous RAFT polymerization was used to prepare a series of hydrophilic-block-cationic copolymers in which the cationic block statistically incorporates increasing amounts of neutral, hydrophilic monomer such that the number of cationic groups remains unchanged but the cationic charge density is diluted along the polymer backbone. Reduced charge density decreases the electrostatic binding strength between copolymers and siRNA with the goal of improving siRNA release after targeted cellular delivery. However, lower binding strength resulted in decreased transfection and RNA interference pathway activation, leading to reduced gene knockdown. Enzymatic siRNA degradation studies with BICs indicated lowered binding strength increases susceptibility to RNases, which is the likely cause for poor gene knockdown.

Comments

This is a pre-copyedited, author-produced PDF of an article accepted for publication in Polymer Chemistry following peer review. The version of record is available online at: 10.1039/C6PY01048B.

Publication Title

Polymer Chemistry

Volume

7

Issue

39

First Page

6044

Last Page

6054

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