Isolation and Characterization of the Microsatellite Loci for the Blackstripe Topminnow Fundulus notatus and Their Variability in Two Closely Related Species

Authors

Kevin A. Feldheim, Pritzker Laboratory for Molecular Systematics and Evolution, The Field MuseumFollow
Brian Kreiser, University of Southern MississippiFollow
B. Schmidt, University of Southern MississippiFollow
David D. Duvernell, Southern Illinois UniversityFollow
Jacob F. Schaefer, University of Southern MississippiFollow

Document Type

Article

Publication Date

11-2014

Department

Biological Sciences

School

Biological, Environmental, and Earth Sciences

Abstract

A total of 17 polymorphic microsatellite loci were isolated from the blackstripe topminnow Fundulus notatus. In a sample of 29 individuals, these loci were found to possess two to 19 alleles with expected heterozygosity values ranging from 0·212 to 0·919 and all but one of the loci conformed to Hardy–Weinberg equilibrium expectations. Many of these loci were polymorphic in the closely related species Fundulus olivaceus and Fundulus euryzonus providing a set of markers that should prove useful in future ecological and evolutionary studies of members of this species complex.

Publication Title

Journal of Fish Biology

Volume

85

Issue

5

First Page

1726

Last Page

1732

Recommended Citation

Feldheim, K. A., Kreiser, B., Schmidt, B., Duvernell, D. D., Schaefer, J. F. (2014). Isolation and Characterization of the Microsatellite Loci for the Blackstripe Topminnow Fundulus notatus and Their Variability in Two Closely Related Species. Journal of Fish Biology, 85(5), 1726-1732.
Available at: https://aquila.usm.edu/fac_pubs/15576