Pseudomonas synxantha 2-79 Transformed With Pyrrolnitrin Biosynthesis Genes Has Improved Biocontrol Activity Against Soilborne Pathogens of Wheat and Canola

Document Type

Article

Publication Date

5-2020

Department

Biological Sciences

School

Biological, Environmental, and Earth Sciences

Abstract

A four-gene operon (prnABCD) from Pseudomonas protegens Pf-5 encoding the biosynthesis of the antibiotic pyrronitrin (Prn) was introduced into P. synxantha (formerly P. fluorescens) 2-79, an aggressive root colonizer of both dryland and irrigated wheat roots that naturally produces the antibiotic phenazine-1-carboxylic acid (PCA) and suppresses both take-all and Rhizoctonia root rot of wheat. Recombinant strains ZHW15 and ZHW25 produced both antibiotics and maintained population sizes in the rhizosphere of wheat that were comparable to those of strain 2-79. The recombinant strains inhibited in vitro the wheat pathogens Rhizoctonia solani AG-8 and AG-2-1, Gaeumannomyces graminis var. tritici, Sclereotinia sclerotiorum, Fusaruim culmorum, and F. pseudograminearum significantly more than did strain 2-79. Both the wild-type and recombinant strains were equally inhibitory of Pythium ultimum. When applied as a seed treatment the recombinant strains suppressed take-all, Rhizoctonia root rot of wheat and Rhizoctonia root and stem rot of canola significantly better than did wild-type strain 2-79.

Publication Title

Phytopathology

Volume

110

Issue

5

First Page

1010

Last Page

1017

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