Ascorbate: A biomarker of herbicide stress in wetland plants

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Marine Science


In laboratory exposures of wetland plants to low herbicide levels (<0.1 mu g/mL), some plants showed increased total ascorbic acid suggesting a stimulatory effect on ascorbic acid synthesis occurred; at higher herbicide concentrations (greater than or equal to 0.1 mu g/mL) a notable decline in total ascorbic acid and increase in the oxidized form, dehydroascorbic acid occurred. Vigna luteola and Sesbania vesicaria were exposed fur 7 and 21 days respectively to atrazine (0.05 to 1 mu g/mL); Spartina alterniflora 28 days at 0.1 mu g/mL trifluralin; Hibiscus moscheutos 14 days at 0.1 and 1 mu g/mL metolachlor in fresh and brackish water. The greatest increase following low dosage occurred with S. alterniflora, increasing from <600 mu g/g wet wt. total ascorbic acid to >1000 mu g/g. Ascorbic acid may be a promising biomarker of estuarine plants exposed to herbicide runoff stimulation of ascorbic acid synthesis may enable some wetland plants used in phytoremediation to cope with low levels of these compounds.

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ACS Symposium Series



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