Title

Repair Mechanisms of UV-Induced DNA Damage in Soybean Chloroplasts

Document Type

Article

Publication Date

12-1-1995

Department

Chemistry and Biochemistry

Abstract

In order to better understand the biochemical mechanisms of DNA metabolism in chloroplasts, repair of UV induced plastome damage in vivo was determined by exposure of soybean suspension cells to UV light and subsequent quantitation of the damage remaining in nuclear and chloroplast encoded genes with time by quantitative polymerase chain reaction (QPCR). The kinetics of damage rapir in the nuclear rbcS gene suggest that photoreactivation and dark mechanisms are active, while for the plastome encoded psbA gene only a light-dependent repair process was detected which is considerably slower than would be expected for photolyase-mediated photoreactivation.

Publication Title

Plant Molecular Biology

Volume

29

Issue

6

First Page

1267

Last Page

1277