Procedure for Selecting Monoclonal Antibodies for Use in a Ligand Displacement Assay of Serum Antibody Levels
Chemistry and Biochemistry
Monoclonal antibodies for use in a ligand displacement assay were selected for specificity and affinity/avidity properties that result in their release and displacement in the presence of specific sample antibody but not in the presence of antibodies against other antigens. A screening process is described which involves measurement of displacement of antibody by an ELISA procedure using an enzyme labeled anti-immunoglobulin, providing a means of demonstrating usefulness of a candidate antibody in a ligand displacement format without necessitating the production of enzyme conjugates of each candidate antibody to be screened. The procedure was used to screen a set of eleven monoclonal antibodies (initially selected for anti-Trichinella spiralis specificity by conventional screening methods), and successfully discriminated between antibodies which were useful in the ligand displacement format and those which were not.
Journal of Immunoassay
Voss, P. G.,
Douglas, W. L.,
Toom, P. M.
(1991). Procedure for Selecting Monoclonal Antibodies for Use in a Ligand Displacement Assay of Serum Antibody Levels. Journal of Immunoassay, 12(1), 125-144.
Available at: http://aquila.usm.edu/fac_pubs/6931