Photosystem 2 Remains Capable of Oxygen Evolution After Losing 1/4 of its Initial Manganese Content
Chemistry and Biochemistry
Mathematics and Natural Sciences
Photosystem 2 (PS 2) membranes were treated with CaCl2 to remove extrinsic polypeptides. After resuspension in a chloride-free buffer, manganese was extracted by leaching or with EDTA. Measurements made using three different methods (electron paramagnetic resonance, kinetics, and direct Mn analysis) agreed that one-fourth of the initial Mn content was lost and that the membrane preparation remained homogenous. Upon readdition of extrinsic polypeptides, the Mn-depleted membranes were capable of oxygen evolution. Thus not all the Mn in PS 2 is necessary for the oxygen evolution function.
(1991). Photosystem 2 Remains Capable of Oxygen Evolution After Losing 1/4 of its Initial Manganese Content. Photosynthetica, 25(4), 631-638.
Available at: https://aquila.usm.edu/fac_pubs/7156