Novel Synthesis of Various Orthogonally Protected C^α-Methyllysine Analogues and Biological Evaluation of a Vapreotide Analogue Containing (S)-α-Methyllysine

Authors

Souvik Banerjee, University of Southern Mississippi
Walker J. Wiggins, University of Southern Mississippi
Jessie L. Geoghegan, University of Southern Mississippi
Catherine T. Anthony, LSU Health Science Center
Eugene A. Woltering, LSU Health Science Center
Douglas S. Masterson, University of Southern MississippiFollow

Document Type

Article

Publication Date

10-7-2013

Department

Chemistry and Biochemistry

School

Mathematics and Natural Sciences

Abstract

Prochiral malonic diesters containing a quaternary carbon center have been successfully transformed into a diverse set of ^tBoc-Fmoc-α^2,2-methyllysine-OH analogues through chiral malonic half-ester intermediates obtained via enzymatic (Pig Liver Esterase, PLE) hydrolysis. The variety of chiral half-ester intermediates, which vary from 1 to 6 methylene units in the side chain, are achieved in moderate to high optical purity and in good yields. The PLE hydrolysis of malonic diesters with various side chain lengths appears to obey the Jones's PLE model according to the stereochemical configurations of the resulting chiral half-esters. The established synthetic strategy allows the construction of both enantiomers of α^2,2-methyllysine analogues, and a (S)-β^2,2-methyllysine analogue from a common synthon by straightforward manipulation of protecting groups. Two different straightforward and cost effective synthetic strategies are described for the synthesis of α^2,2-methyllysine analogues. The described strategies should find significant usefulness in preparing novel peptide libraries with unnatural lysine analogues. A Vapreotide analogue incorporating (S)-α^2,2-methyllysine was prepared. However, the Vapreotide analogue with (S)-α-methyl-α-lysine is found to lose its specific binding to somatostatin receptor subtype 2 (SSTR2).

Publication Title

Organic & Biomolecular Chemistry

Volume

11

Issue

37

First Page

6307

Last Page

6319

Recommended Citation

Banerjee, S., Wiggins, W. J., Geoghegan, J. L., Anthony, C. T., Woltering, E. A., Masterson, D. S. (2013). Novel Synthesis of Various Orthogonally Protected C^α-Methyllysine Analogues and Biological Evaluation of a Vapreotide Analogue Containing (S)-α-Methyllysine. Organic & Biomolecular Chemistry, 11(37), 6307-6319.
Available at: https://aquila.usm.edu/fac_pubs/7842