Degradation of Pyrimidine Ribonucleosides by Pseudomonas aeruginosa
Pyrimidine ribonucleoside degradation in the human pathogen Pseudomonas aeruginosa ATCC 15692 was investigated. Either uracil, cytosine, 5-methylcytosine, thymine, uridine or cytidine supported P. aeruginosa growth as a nitrogen source when glucose served as the carbon source. Using thin-layer chromatographic analysis, the enzymes nucleoside hydrolase and cytosine deaninase were shown to be active in ATCC 15692. Compared to (NH4)2SO4-grown cells, nucleoside hydrolase activity in ATCC 15692 approximately doubled after growth on 5-methylcytosine as a nitrogen source while its cytosine deaminase activity increased several-fold after growth on the pyrimidine bases and ribonucleosides examined as nitrogen sources. Regulation at the level of protein synthesis by 5-methylcytosine was indicated for nucleoside hydrolase and cytosine deaminase in P. aeruginosa.
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
West, T. P.
(1996). Degradation of Pyrimidine Ribonucleosides by Pseudomonas aeruginosa. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 69(4), 331-335.
Available at: https://aquila.usm.edu/fac_pubs/8729