Degradation of Pyrimidine Ribonucleosides by Pseudomonas aeruginosa

Authors

Thomas P. West, University of Southern MississippiFollow

Document Type

Article

Publication Date

5-1-1996

Department

Biological Sciences

School

Biological, Environmental, and Earth Sciences

Abstract

Pyrimidine ribonucleoside degradation in the human pathogen Pseudomonas aeruginosa ATCC 15692 was investigated. Either uracil, cytosine, 5-methylcytosine, thymine, uridine or cytidine supported P. aeruginosa growth as a nitrogen source when glucose served as the carbon source. Using thin-layer chromatographic analysis, the enzymes nucleoside hydrolase and cytosine deaninase were shown to be active in ATCC 15692. Compared to (NH₄)₂SO₄-grown cells, nucleoside hydrolase activity in ATCC 15692 approximately doubled after growth on 5-methylcytosine as a nitrogen source while its cytosine deaminase activity increased several-fold after growth on the pyrimidine bases and ribonucleosides examined as nitrogen sources. Regulation at the level of protein synthesis by 5-methylcytosine was indicated for nucleoside hydrolase and cytosine deaminase in P. aeruginosa.

Publication Title

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology

Volume

69

Issue

4

First Page

331

Last Page

335

Recommended Citation

West, T. P. (1996). Degradation of Pyrimidine Ribonucleosides by Pseudomonas aeruginosa. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 69(4), 331-335.
Available at: https://aquila.usm.edu/fac_pubs/8729