Synthesis of Photolabile Transcription Initiators and Preparation of Photocleavable Functional RNA by Transcription
Document Type
Article
Publication Date
7-1-2012
Department
Chemistry and Biochemistry
School
Mathematics and Natural Sciences
Abstract
Two new photolabile adenosine-containing transcription initiators with terminal thiol and amino functionalities are chemically synthesized. Transcription in the presence of the transcription initiators under the T7 phi2.5 promoter produces 50 thiol- and amino-functionalized RNA conjugated by a photocleavable (PC) linker. Further RNA functionalization with biotin may be achieved through acyl transfer reactions from either biotinyl AMP to the RNA thiol group or biotin NHS to the RNA amino group. Photocleavage of the PC linker displays relatively fast kinetics with a half-life of 4-5 min. The availability of these transcription initiators makes new photolabile RNA accessible for affinity purification of RNA, in vitro selection of functional RNAs, and functional RNA caging. (C) 2012 Elsevier Ltd. All rights reserved.
Publication Title
Biorganic and Medicinal Chemistry Letters
Volume
22
Issue
13
First Page
4254
Last Page
4258
Recommended Citation
Huang, F.,
Shi, Y.
(2012). Synthesis of Photolabile Transcription Initiators and Preparation of Photocleavable Functional RNA by Transcription. Biorganic and Medicinal Chemistry Letters, 22(13), 4254-4258.
Available at: https://aquila.usm.edu/fac_pubs/8872