Date of Award
Honors College Thesis
Mohamed O. Elasri, Ph.D.
Staphylococcus aureus is an important human pathogen that causes a wide variety of diseases. Many S. aureus strains have emerged which are resistant to the penicillin class of antibiotics. Of primary importance is methicillin-resistant S. aureus (MRSA), which has cause frequent hospitalizations due to infections. In the past, MRSA was typically confined to hospital settings, but recently, community-associated MRSA (CA-MRSA) have been reported. CA-MRSA poses a major public health threat because of increased virulence and success in infecting otherwise healthy individuals. Previously we discovered a gene, msa, which plays a critical role in biofilm formation and regulation of the disease process. Recent studies indicate that msa is part of a three open reading frame operon and that the upstream neighboring genes may play a role in the regulation of the msa operon. In this study, we investigated the possibility that genes 1294-1298 regulate virulence factors of S. aureus. We constructed a 1294-1298 mutant in CA-MRSA USA300 strain LAC using the allelic replacement vector pKOR1 and found that it produced a weaker biofilm in addition to increased autolysis, protease production, pigmentation production, hemolysin production and lipase production-all indicators that genes 1294-1298 play a role in the virulence of S. aureus. We hope to explore the possibility of exploring the regulatory network of the msa operon and its neighboring genes and exploit them as a target for therapy for recalcitrant staph infections.
Copyright for this thesis is owned by the author. It may be freely accessed by all users. However, any reuse or reproduction not covered by the exceptions of the Fair Use or Educational Use clauses of U.S. Copyright Law or without permission of the copyright holder may be a violation of federal law. Contact the administrator if you have additional questions.
Dawoud, Reem A., "Deletion of Putative msa Operon Gene Fragment and Its Phenotypic Characterization in Community Acquired-Methicillin Resistant Staphylococcus aureus USA300 strain LAC" (2013). Honors Theses. 117.