Date of Award

5-1-2014

Degree Type

Honors College Thesis

Department

Biological Sciences

First Advisor

Anthony Bell, Ph.D.

Abstract

Small molecules can be used promote the formation or assembly of four-way DNA junctions (4WJs). A series of small molecules were evaluated with four-way and three-way junctions (3WJs). From these assays, a ruthenium biphenyl compound [Ru(byp)2] generated the best results. 4WJs and 3WJs formed stable complexes when incubated with Ru(byp)2 in the presence of MgCl2 – conditions that are more similar to physiological conditions. Future research will focus on measuring the thermostability of Ru(byp)2-stablized 4WJs via circular dichroism (CD) and UV spectroscopy. The long-term goal of this strategy is to use Ru(byp)2 or alternate small molecules to target/stall DNA replication of cancer cells.

Available for download on Tuesday, March 02, 2219

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