Date of Award

Spring 5-11-2012

Degree Type

Honors College Thesis


Chemistry and Biochemistry

First Advisor

Faqing Huang

Advisor Department

Chemistry and Biochemistry


As gene therapies continue to be developed, the role played by siRNA technologies in anti-cancer regimens seems likely to increase. And, as siRNA technologies continue to be studied, the use of gold nanoparticle conjugate systems to deliver siRNA in vivo will proliferate as well. In light of this trend in cancer therapeutics, this project aimed to design a AuNP carrier for siRNA. With the goal of creating an efficient base from which effective siRNA-AuNP conjugates could be synthesized, the development of C12PEI coated AuNPs was closely investigated. Toward this end, AuNP- C12PEI conjugates were synthesized with an average diameter of 25 nm, an average zeta potential of +58 mV, and an average of 258 C12PEI units per AuNP. Additionally, C12PEI-AuNPs were successfully conjugated with tRNA in preparation for siRNA binding studies.