Title

INHIBITION OF CYSTEINE PROTEINASES BY CARICA-PAPAYA CYSTATIN PRODUCED IN ESCHERICHIA-COLI

Document Type

Article

Publication Date

9-11-1995

Department

Chemistry and Biochemistry

Abstract

A papaya cystatin (Cst)-encoding cDNA clone was isolated from a papaya leaf cDNA library and the active protein produced in Escherichia coli. The amino-acid sequence reveals a protein of 11262 Da with over 40% identity to other published plant Cst. Unique features of the papaya Cst include a single Cys residue, variation in the papain-binding region, and the first reported inhibition of papaya proteinase IV by a Cst.

Publication Title

GENE

Volume

162

Issue

2

First Page

221

Last Page

224