Inhibition of Cysteine Proteinases by Carica-Papaya Cystatin Produced in Escherichia coli
Chemistry and Biochemistry
A papaya cystatin (Cst)-encoding cDNA clone was isolated from a papaya leaf cDNA library and the active protein produced in Escherichia coli. The amino-acid sequence reveals a protein of 11262 Da with over 40% identity to other published plant Cst. Unique features of the papaya Cst include a single Cys residue, variation in the papain-binding region, and the first reported inhibition of papaya proteinase IV by a Cst.
Bateman, R. C.
(1995). Inhibition of Cysteine Proteinases by Carica-Papaya Cystatin Produced in Escherichia coli. Gene, 162(2), 221-224.
Available at: http://aquila.usm.edu/fac_pubs/5955