Inhibition of Cysteine Proteinases by Carica-Papaya Cystatin Produced in Escherichia coli

Document Type

Article

Publication Date

9-11-1995

Department

Chemistry and Biochemistry

School

Mathematics and Natural Sciences

Abstract

A papaya cystatin (Cst)-encoding cDNA clone was isolated from a papaya leaf cDNA library and the active protein produced in Escherichia coli. The amino-acid sequence reveals a protein of 11262 Da with over 40% identity to other published plant Cst. Unique features of the papaya Cst include a single Cys residue, variation in the papain-binding region, and the first reported inhibition of papaya proteinase IV by a Cst.

Publication Title

Gene

Volume

162

Issue

2

First Page

221

Last Page

224

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