Document Type
Article
Publication Date
8-24-2016
Department
Biological Sciences
Abstract
Zika virus (ZIKV) is a globally emerging mosquito-transmitted flavivirus that can cause severe fetal abnormalities, including microcephaly. As such, highly sensitive, specific, and cost-effective diagnostic methods are urgently needed. Here, we report a novel electrogenerated chemiluminescence (ECL)-based immunoassay for ultrasensitive and specific detection of ZIKV in human biological fluids. We loaded polystyrene beads (PSB) with a large number of ECL labels and conjugated them with anti-ZIKV monoclonal antibodies to generate anti-ZIKV-PSBs. These anti-ZIKV-PSBs efficiently captured ZIKV in solution forming ZIKV-anti-ZIKV-PSB complexes, which were subjected to measurement of ECL intensity after further magnetic beads separation. Our results show that the anti-ZIKV-PSBs can capture as little as 1 PFU of ZIKV in 100 μl of saline, human plasma, or human urine. This platform has the potential for development as a cost-effective, rapid and ultrasensitive assay for the detection of ZIKV and possibly other viruses in clinical diagnosis, epidemiologic and vector surveillance, and laboratory research.
Publication Title
Scientific Reports
Volume
6
First Page
1
Last Page
11
Recommended Citation
Acharya, D.,
Bastola, P.,
Le, L.,
Paul, A. M.,
Fernandez, E.,
Diamond, M. S.,
Miao, W.,
Bai, F.
(2016). An Ultrasensitive Electrogenerated Chemiluminescence-Based Immunoassay for Specific Detection of Zika Virus. Scientific Reports, 6, 1-11.
Available at: https://aquila.usm.edu/fac_pubs/15114
Comments
Publisher Version: https://doi.org/10.1038/srep32227