Document Type
Article
Publication Date
11-1-2008
Department
Coastal Sciences, Gulf Coast Research Laboratory
Abstract
A central, unresolved problem of DNA microarray technology is the interpretation of different signal intensities from multiple probes targeting the same transcript. We propose a competitive hybridization model for DNA microarray hybridization. Our model uses a probe-specific dissociation constant that is computed with current nearest neighbor model and existing parameters, and only four global parameters that are fitted to Affymetrix Latin Square data. This model can successfully predict signal intensities of individual probes, therefore makes it possible to quantify the absolute concentration of targets. Our results offer critical insights into the design and data interpretation of DNA microarrays.
Publication Title
Nucleic Acids Research
Volume
36
Issue
20
First Page
6585
Last Page
6591
Recommended Citation
Li, S.,
Pozhitkov, A.,
Brouwer, M.
(2008). A Competitive Hybridization Model Predicts Probe Signal Intensity On High Density DNA Microarrays. Nucleic Acids Research, 36(20), 6585-6591.
Available at: https://aquila.usm.edu/fac_pubs/1576
Comments
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582621/pdf/gkn740.pdf