Self-Assembling PCL-PAMAM Linear Dendritic Block Copolymers (LDBCs) for Bioimaging and Phototherapeutic Applications

Authors

Indika Chandrasiri, University of MississippiFollow
Daniel G. Abebe, University of Mississippi
Mahesh Loku Yaddehige, University of Mississippi
Jon Steven Dal Williams, University of Mississippi
Mohammad Farid Zia, University of Southern MississippiFollow
Austin Dorris, University of MississippiFollow
Abigail Barker, University of Mississippi
Briana L. Simms, University of Mississippi
Azaziah Parker, University of Mississippi
Bhavani Prasad Vinjamuri, University of Mississippi
Ngoc Le, University of Mississippi
Jacqueline N. Gayton, University of MississippiFollow
Mahavir Bhupal Chougule, University of Mississippi
Nathan I. Hammer, University of MississippiFollow
Alex Flynt, University of Southern MississippiFollow
Jared H. Delcamp, University of MississippiFollow
Davita L. Watkins, University of MississippiFollow

Document Type

Article

Publication Date

9-21-2020

Department

Chemistry and Biochemistry

School

Mathematics and Natural Sciences

Abstract

Copyright © 2020 American Chemical Society. This study represents a successful approach toward employing polycaprolactone-polyamidoamine (PCL-PAMAM) linear dendritic block copolymer (LDBC) nanoparticles as small-molecule carriers in NIR imaging and photothermal therapy. A feasible and robust synthetic strategy was used to synthesize a library of amphiphilic LDBCs with well-controlled hydrophobic-to-hydrophilic weight ratios. Systems with a hydrophobic weight ratio higher than 70% formed nanoparticles in aqueous media, which show hydrodynamic diameters of 51.6 and 96.4 nm. These nanoparticles exhibited loading efficiencies up to 21% for a hydrophobic molecule and 64% for a hydrophilic molecule. Furthermore, successful cellular uptake was observed via trafficking into endosomal and lysosomal compartments with an encapsulated NIR theranostic agent (C3) without inducing cell death. A preliminary photothermal assessment resulted in cell death after treating the cells with encapsulated C3 and exposing them to NIR light. The results of this work confirm the potential of these polymeric materials as promising candidates in theranostic nanomedicine.

Publication Title

ACS Applied Bio Materials

Volume

3

Issue

9

First Page

5664

Last Page

5677

Recommended Citation

Chandrasiri, I., Abebe, D., Loku Yaddehige, M., Williams, J., Zia, M., Dorris, A., Barker, A., Simms, B., Parker, A., Vinjamuri, B., Le, N., Gayton, J., Chougule, M., Hammer, N., Flynt, A., Delcamp, J., Watkins, D. (2020). Self-Assembling PCL-PAMAM Linear Dendritic Block Copolymers (LDBCs) for Bioimaging and Phototherapeutic Applications. ACS Applied Bio Materials, 3(9), 5664-5677.
Available at: https://aquila.usm.edu/fac_pubs/18218