Aqueous RAFT Synthesis of Low Molecular Weight Anionic Polymers for Determination of Structure/Binding Interactions with Gliadin
Document Type
Article
Publication Date
8-1-2020
School
Polymer Science and Engineering
Abstract
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Gliadin, a component of gluten and a known epitope, is implicated in celiac disease (CeD) and results in an inflammatory response in CeD patients when consumed. Acrylamide-based polyelectrolytes are employed as models to determine the effect of molecular weight and pendent group on non-covalent interaction modes with gliadin in vitro. Poly(sodium 2-acrylamido-2-methylpropane sulfonate) and poly(sodium 3-methylpropyl-3-butanoate) are synthesized via aqueous reversible addition fragmentation chain transfer (aRAFT) polymerization and characterized by gel permeation chromatography-multiangle laser light scattering. The polymer/gliadin blends are examined via circular dichroism, zeta potential measurements, 8-anilinonaphthalene-1-sulfonic acid fluorescence spectroscopy, and dynamic light scattering. Acrylamide polymers containing strong anionic pendent groups have a profound effect on gliadin secondary structure and solution behavior below the isoelectric point, while polymers containing hydrophobic character only have a minor impact. The polymers have little effect on gliadin secondary structure and solution behavior at the isoelectric point.
Publication Title
Macromolecular Bioscience
Volume
20
Issue
8
Recommended Citation
Bristol, A.,
Carpenter, B.,
Davis, A.,
Kemp, L.,
Rangachari, V.,
Karim, S.,
Morgan, S.
(2020). Aqueous RAFT Synthesis of Low Molecular Weight Anionic Polymers for Determination of Structure/Binding Interactions with Gliadin. Macromolecular Bioscience, 20(8).
Available at: https://aquila.usm.edu/fac_pubs/18246