Development and Characterization of Eighty-Four Microsatellite Markers For the Red Snapper (Lutjanus campechanus) Using Illumina Paired-End Sequencing
Document Type
Article
Publication Date
6-20-2014
Department
Coastal Sciences, Gulf Coast Research Laboratory
School
Ocean Science and Engineering
Abstract
Eighty-four microsatellite loci were isolated from red snapper, Lutjanus campechanus, using Illumina paired-end sequencing and a direct ‘Seq-to SSR’ approach. The number of alleles per locus ranged from 7 to 30 and estimates of expected heterozygosity ranged from 0.466 to 0.967 (n = 30). Genotype frequencies at 7 loci deviated significantly from Hardy-Weinberg expectations; the departure at 4 of these loci likely reflects the segregation of null alleles. The markers characterized in this work will be applied to linkage mapping and genetic studies involved in the domestication and stock enhancement of red snapper. Development of the 84 microsatellites required screening approximately 1.5 M paired-end reads, highlighting the potential of this method for rapid and cost-effective development of new homologous microsatellites in emerging aquaculture species.
Publication Title
Aquaculture
Volume
430
First Page
128
Last Page
132
Recommended Citation
Norrell, A.,
Crawley, D.,
Jones, K.,
Saillant, E.
(2014). Development and Characterization of Eighty-Four Microsatellite Markers For the Red Snapper (Lutjanus campechanus) Using Illumina Paired-End Sequencing. Aquaculture, 430, 128-132.
Available at: https://aquila.usm.edu/fac_pubs/19913