Effects of Nanoaggregation On Isoindigo-Based Fluorophores For Near-Infrared Bioimaging Applications
Document Type
Article
Publication Date
4-27-2022
Department
Biological Sciences
School
Biological, Environmental, and Earth Sciences
Abstract
In this work, we have taken a donor–acceptor–donor (D–A–D) fluorophore (II-EDOT-TPA) and encapsulated it using a linear dendritic block copolymer (LDBC). In parallel, a polyethylene glycol derivative (PEG-II-EDOT-TPA) was synthesized. The self-assembly and colloidal properties of both nanoaggregates were comparatively assessed. Photophysical and morphological characterization of the LDBC encapsulated II-EDOT-TPA and PEG-II-EDOT-TPA nanoaggregates was performed, which showed the photophysical and morphological properties differed greatly when comparing the two. Both nanoaggregate types were incubated with HEK-293 cells in order to measure cell viability and perform confocal fluorescence microscopy. Minimal cytotoxicity values (<20%) were seen with the two nanoaggregate forms, while both types of nanoaggregates were found to accumulate into the lysosomes of the HEK-293 cells. This work provides fascinating insights into NIR fluorophore design and methods to effectively alter the photophysical and morphological properties of the nanoaggregates for bio-imaging purposes.
Publication Title
Molecular Systems Design & Engineering
Recommended Citation
Sparks, N. E.,
Dorris, A.,
Vijayan, S. M.,
Chandrasiri, I.,
Zia, M. F.,
Flynt, A.,
Hammer, N. I.,
Watkins, D. L.
(2022). Effects of Nanoaggregation On Isoindigo-Based Fluorophores For Near-Infrared Bioimaging Applications. Molecular Systems Design & Engineering.
Available at: https://aquila.usm.edu/fac_pubs/19936