Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection

Authors

Farzana Nazneen, University of Southern MississippiFollow
Fengwei Bai, University of Southern MississippiFollow

Document Type

Book Chapter

Publication Date

11-5-2022

Department

Biological Sciences

School

Biological, Environmental, and Earth Sciences

Abstract

Primary neurons are very valuable cells to study the pathogenesis of neurotropic viruses, such as West Nile virus. The mouse primary neurons can be used to assess viral infection profiles and cellular immune responses to a viral infection. However, successful isolation and culture of mouse neurons can be challenging. Here, we report a step-by-step method to prepare a primary neuron culture from adult mice.

Publication Title

West Nile Virus

First Page

23

Last Page

31

Recommended Citation

Nazneen, F., Bai, F. (2022). Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection. West Nile Virus, 23-31.
Available at: https://aquila.usm.edu/fac_pubs/20486