Isolation of Murine Bone Marrow-Derived Neutrophils for Infection Modeling
Document Type
Book Chapter
Publication Date
11-5-2022
Department
Biological Sciences
School
Biological, Environmental, and Earth Sciences
Abstract
West Nile virus (WNV) is a single-stranded-RNA flavivirus that can cause neurological illnesses. The ability of WNV to cause neurological illnesses is dependent on the virus’ ability to gain access into the brain. However, the mechanisms by which WNV enters the brain are elusive, with evidence that neutrophils act as vehicles for viral delivery. To determine the role of neutrophils in WNV central nervous system delivery, modified protocols for the isolation and migration of neutrophils from mouse bone marrow for in vitro WNV infection modeling is described.
Publication Title
West Nile Virus
First Page
33
Last Page
40
Recommended Citation
Duty, L.,
Paul, A. M.
(2022). Isolation of Murine Bone Marrow-Derived Neutrophils for Infection Modeling. West Nile Virus, 33-40.
Available at: https://aquila.usm.edu/fac_pubs/20499
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