Detoxification of Copper and Chromium Via Dark Hydrogen Fermentation of Potato Waste by Clostridium butyricum Strain 92

Authors

Vira Hovorukha, Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of UkraineFollow
Olesia Havryliuk, Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of UkraineFollow
Galina Gladka, Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of UkraineFollow
Antonina Kalinichenko, University of OpoleFollow
Monika Sporek, University OpoleFollow
Jan Stebila, Matej Bel UniversityFollow
Dmitri Mavrodi, University of Southern MississippiFollow
Ruslan Mariychuk, Prešov University in PrešovFollow
Oleksandr Tashyrev, Zabolotny Institute of Microbiology and Virology of the National Acadmey of Sciences of UkraineFollow

Document Type

Article

Publication Date

1-17-2022

Department

Biological Sciences

School

Biological, Environmental, and Earth Sciences

Abstract

The accumulation of various types of waste containing both organic and inorganic metal-containing compounds is extremely hazardous for living organisms. The possibility of polymer degradation, biohydrogen synthesis, and metal detoxification via the dark fermentation of model potato waste was investigated. For this purpose, the strict anaerobic strain was isolated and identified as Clostridium butyricum. The high efficiency of dark hydrogen fermentation of potatoes with yield of hydrogen in 85.8 ± 15.3 L kg⁻¹ VS_potato was observed. The copperand chromium salts solutions were added to the culture fluid to obtain the concentrations of 50, 100, and 200 mg L⁻¹ Cu(II) and Cr(VI) in the active phase of growth (19 h of cultivation). Metals at a concentration of 200 mg L⁻¹ inhibited the fermentation process the most. The hydrogen yield decreased in 7.2 and 3.6 times to 11.9 ± 2.1 and 23.8 ± 5.6 L kg⁻¹ VSpotato in the presence of 200 mg L⁻¹ Cu(II) and Cr(VI), respectively. The efficiencies of the chromium bioremoval in all variants of the experiment were 100%, and those of copper bioremoval were about 90%. A pure culture of strict anaerobes Clostridium butyricum strain 92 was used for the first time for the detoxification of metals. The presented results confirmed the possibility of this promising strain application for industrial H₂ production and the bioremediation of contaminated sites.

Publication Title

Processes

Volume

10

Issue

1

Recommended Citation

Hovorukha, V., Havryliuk, O., Gladka, G., Kalinichenko, A., Sporek, M., Stebila, J., Mavrodi, D., Mariychuk, R., Tashyrev, O. (2022). Detoxification of Copper and Chromium Via Dark Hydrogen Fermentation of Potato Waste by Clostridium butyricum Strain 92. Processes, 10(1).
Available at: https://aquila.usm.edu/fac_pubs/20765