Dissecting SNARE-Mediated Exocytosis in RBL-2H3 Mast Cells

Authors

Pratikshya Adhikari, The University of North Carolina at Chapel HillFollow
Hao Xu, University of Southern MississippiFollow

Document Type

Book Chapter

Publication Date

1-14-2025

Department

Biological Sciences

School

Biological, Environmental, and Earth Sciences

Abstract

SNARE-dependent mast cell (MC) exocytosis causes the release of a wide variety of mediators with important physiological/pathological consequences. Unlike synaptic transmission in the brain, which relies primarily on one set of exocytic SNAREs (i.e., Syntaxin1, SNAP-25, and VAMP2), MCs produce a multitude of exocytic SNAREs that can form a minimum of 8 distinct sets of fusogenic trans-SNARE complexes. Here we describe the genetic approaches we have developed to dissect the specific roles of these SNAREs in RBL-2H3 cells, a widely utilized model for studying MC signaling and exocytosis.

Publication Title

Methods in Molecular Biology

Volume

2887

First Page

149

Last Page

166

Recommended Citation

Adhikari, P., Xu, H. (2025). Dissecting SNARE-Mediated Exocytosis in RBL-2H3 Mast Cells. Methods in Molecular Biology, 2887, 149-166.
Available at: https://aquila.usm.edu/fac_pubs/21821