Identification of Alpha-Gal glycolipids in saliva of Lone-Star Tick (Amblyomma americanum)
Document Type
Article
Publication Date
11-1-2024
School
Biological, Environmental, and Earth Sciences
Abstract
Alpha-Gal Syndrome (AGS) is a delayed allergic reaction triggered by IgE antibodies targeting galactose-α-1,3-galactose (α-gal), prevalent in red meat. Its global significance has increased, with over 450,000 estimated cases in the United States alone. AGS is linked to tick bites, causing sensitization and elevated α-gal specific IgE levels. However, the precise mechanisms and tick intrinsic factors contributing to AGS development post-tick bites remain unclear. This study aims to characterize the alpha-gal conjugated lipid antigens in Amblyomma americanum (Am. americanum) salivary glands and saliva. Nanospray ionization mass spectrometry (NSI-MS) analysis revealed the identification of α-gal bound lipid antigens in Am. americanum saliva. Additionally, the activation of basophils by extracted alpha-gal bound lipids and proteins provides evidence of their antigenic capabilities.
Publication Title
Ticks and Tick Borne Diseases
Volume
15
Issue
6
Recommended Citation
Sharma, S.,
Hussain, S.,
Choudhary, S.,
Commins, S.,
Karim, S.
(2024). Identification of Alpha-Gal glycolipids in saliva of Lone-Star Tick (Amblyomma americanum). Ticks and Tick Borne Diseases, 15(6).
Available at: https://aquila.usm.edu/fac_pubs/22306