Exocrine Pancreatic Carcinogenesis in the Guppy Poecilia reticulata
Document Type
Article
Publication Date
12-10-2002
Department
Marine Science
Abstract
Exocrine pancreatic neoplasms developed in the guppy Poecilia reticulata following exposure to the direct-acting carcinogen methylazoxymethanol acetate (MAM-Ac). Fish 6 to 10 d old were exposed to nominal, non-toxic concentrations of 4 and 10 mg MAM-Ac l(-1) for 2 h and then transferred to carcinogen-free water for grow-out. Whole specimens were sampled monthly up to 9 mo post-exposure to follow the histologic progression of the lesions. No neoplasms occurred in 119 control specimens examined. Pancreatic acinar cell adenomas and carcinomas occurred in 42 of 243 (17%) of the specimens exposed to MAM-Ac. As in earlier studies, specimens exposed to the low MAM-Ac concentration exhibited a higher pancreatic neoplasm incidence (27.8%) than those exposed to the high concentration (7.8%). Acinar cell adenomas accounted for 27 of the 42 neoplasms. Adenomas exhibited a high degree of acinar cell differentiation and some contained foci of atypical acinar cells that were less differentiated and more basophilic than were surrounding adenoma cells. Carcinomas occurred in 15 specimens and exhibited a range of cellular patterns, Although no distant metastases were found, carcinomas tended to invade neighboring tissues and organs, The occurrence of carcinogen-induced pancreatic neoplasms in guppies strengthens the usefulness of small fish species in carcinogen testing and provides an additional model for studying pancreatic neoplasia.
Publication Title
Diseases of Aquatic Organisms
Volume
52
Issue
3
First Page
191
Last Page
198
Recommended Citation
Fournie, J. W.,
Hawkins, W. E.
(2002). Exocrine Pancreatic Carcinogenesis in the Guppy Poecilia reticulata. Diseases of Aquatic Organisms, 52(3), 191-198.
Available at: https://aquila.usm.edu/fac_pubs/3437