The Nuclear Pore Complex Mediates Binding of the Mig1 Repressor to Target Promoters

Authors

Nayan J. Sarma, University of Southern MississippiFollow
Thomas D. Buford, University of Southern MississippiFollow
Terry Haley, University of Southern MississippiFollow
Kellie Barbara-Haley, University of Southern MississippiFollow
George M. Santangelo, University of Southern MississippiFollow
Kristine A. Willis, University of Southern MississippiFollow

Document Type

Article

Publication Date

11-1-2011

Department

Biological Sciences

School

Biological, Environmental, and Earth Sciences

Abstract

All eukaryotic cells alter their transcriptional program in response to the sugar glucose. In Saccharomyces cerevisiae, the best-studied downstream effector of this response is the glucose-regulated repressor Mig1. We show here that nuclear pore complexes also contribute to glucose-regulated gene expression. NPCs participate in glucose-responsive repression by physically interacting with Mig1 and mediating its function independently of nucleocytoplasmic transport. Surprisingly, despite its abundant presence in the nucleus of glucose-grown nup120Δ or nup133Δ cells, Mig1 has lost its ability to interact with target promoters. The glucose repression defect in the absence of these nuclear pore components therefore appears to result from the failure of Mig1 to access its consensus recognition sites in genomic DNA. We propose that the NPC contributes to both repression and activation at the level of transcription.

Publication Title

PLoS One

Volume

6

Issue

11

First Page

1

Last Page

10

Recommended Citation

Sarma, N., Buford, T. D., Haley, T., Barbara-Haley, K., Santangelo, G. M., Willis, K. A. (2011). The Nuclear Pore Complex Mediates Binding of the Mig1 Repressor to Target Promoters. PLoS One, 6(11), 1-10.
Available at: https://aquila.usm.edu/fac_pubs/581