Instability of the ABTS Peroxidase Reaction Product in Biological Buffers
Document Type
Article
Publication Date
8-1-1993
Department
Chemistry and Biochemistry
School
Mathematics and Natural Sciences
Abstract
The commonly used horseradish peroxidase dye substrate, 2,2'-azino-bis(3-ethyl-benzthiazoline-6-sulphonic acid) (ABTS), was found to be unstable under certain conditions after reaction with the peroxidase. A survey of several buffers and pH values showed that the oxidized dye was destabilized by raising the pH or by the use of ''Good'' buffers. It is recommended that use of this dye in a peroxidase-based detection system be confined to acetate buffer at low pH, if possible, and that phosphate or Tris buffers be used if a pH near neutrality is required
Publication Title
Biotechniques
Volume
15
Issue
2
First Page
270
Last Page
&
Recommended Citation
McCoy-Messer, J.,
Bateman, R. C.
(1993). Instability of the ABTS Peroxidase Reaction Product in Biological Buffers. Biotechniques, 15(2), 270-&.
Available at: https://aquila.usm.edu/fac_pubs/6525