Detection of Poly(U) Hybridization Using Azido Modified Poly(A)-Coated Piezoelectric Crystals
Document Type
Article
Publication Date
10-29-1993
Department
Chemistry and Biochemistry
School
Mathematics and Natural Sciences
Abstract
A technique has been developed which uses AT-cut, 9 MHz piezoelectric crystals for the immobilization of single stranded nucleic acids and the subsequent hybridization reactions. This hybridization technique is based on detecting small changes in mass on the surface of a piezoelectric crystal which occur upon immobilizing azido containing probe nucleic acid on the surface and hybridizing complementary target nucleic acid to previously immobilized probe. The immobilized probe could be used for repeated hybridization tests. The hybridization assays were performed in solution and the frequency measurements were performed in the dry state.
Publication Title
Biochemical and Biophysical Research Communications
Volume
196
Issue
2
First Page
858
Last Page
863
Recommended Citation
Campbell, N.,
Evans, J. A.,
Fawcett, N.
(1993). Detection of Poly(U) Hybridization Using Azido Modified Poly(A)-Coated Piezoelectric Crystals. Biochemical and Biophysical Research Communications, 196(2), 858-863.
Available at: https://aquila.usm.edu/fac_pubs/6685