Title

Detection of Poly(U) Hybridization Using Azido Modified Poly(A)-Coated Piezoelectric Crystals

Document Type

Article

Publication Date

10-29-1993

Department

Chemistry and Biochemistry

Abstract

A technique has been developed which uses AT-cut, 9 MHz piezoelectric crystals for the immobilization of single stranded nucleic acids and the subsequent hybridization reactions. This hybridization technique is based on detecting small changes in mass on the surface of a piezoelectric crystal which occur upon immobilizing azido containing probe nucleic acid on the surface and hybridizing complementary target nucleic acid to previously immobilized probe. The immobilized probe could be used for repeated hybridization tests. The hybridization assays were performed in solution and the frequency measurements were performed in the dry state.

Publication Title

Biochemical and Biophysical Research Communications

Volume

196

Issue

2

First Page

858

Last Page

863

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