Has the Importance of Photoautotrophic Picoplankton Been Overestimated

Document Type

Article

Publication Date

3-1-1994

Department

Marine Science

Abstract

Postincubation differential filtration (PIDF), preincubation differential filtration (Pre-IDF), and track autoradiography (TA) were compared for estimating cell-specific and total photo-autotrophic picoplankton production. Experiments were performed in Lakes Michigan and Huron and in the Ful of Mexico. When Synechococcus dominated the photoautotrophic picoplankton community (>70% of total picoplankton abundance), PIDF estimates of cell-specific and total picoplankton production were ~3.0 x (range, 2.0--3.8 x) higher than TA estimates. PreIDF estimates of cell-specific and total picoplankton production, however, were only slightly higher than TA estimates (mean, 1.4 x; range, 1.4--1.5 x). The higher PIDF estimates were attributable to breakage and damage of larger photoautotrophs during postincubation filtration and to retention of this labeled material on the smaller (0.2 µm) pore-size filter. Results from PIDF experiments must be viewed with caution and previous estimates of picoplankto production, cell-specific or total, based solely on PIDF may need to be re-evaluated.

Publication Title

Limnology and Oceanography

Volume

39

Issue

2

First Page

432

Last Page

438

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